D9170
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D9170
Sigma
Dimethyl sulfoxide
PCR Reagent
| Synonym: | DMSO, Methyl sulfoxide |
| CAS Number: | 67-68-5 |
| Linear Formula: | (CH3)2SO |
| Molecular Weight: | 78.13 |
| Beilstein Registry Number: | 506008 |
| EC Number: | 200-664-3 |
| MDL number: | MFCD00002089 |
| PubChem Substance ID: | 24894271 |
Description
| Application | Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR. |
| Caution | Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product. |
Properties
| grade | PCR Reagent |
| vapor density | 2.7 (vs air) |
| vapor pressure | 0.42 mmHg ( 20 °C) |
| autoignition temp. | 573 °F |
| expl. lim. | 42 %± , 63 °F |
| packaging | vial of 1 mL |
| refractive index | n |
| bp | 189 °C(lit.) |
| mp | 16-19 °C(lit.) |
| density | 1.10 g/mL(lit.) |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
| WGK Germany | 1 |
| RTECS | PV6210000 |
| UEL | 42.00%(V) |
| LEL | 3.50%(V) |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
References
| reference | Cheng, S., et al., Effective amplification of long targets from cloned inserts and human genomic DNA. Proc. Natl. Acad. Sci. U. S. A. 91, 5695-5699, (1994) Abstract |
| Winship, P.R., et al., An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Res. 17, 1266, (1989) Abstract | |
| Merck | Merck 13,3285 |
| Beilstein | Beil. 1,IV,1277 |
| Fieser | Fieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149 |
| Aldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A |
